Evaluation of microleakage in class v cavities prepared by different caries removal methods.

The purpose of this study was to comparatively evaluate the effects of different caries removal methods on microleakage success of class V adhesive restorations by means of light microscopy (stereomicroscope) and scanning electron microscopy (SEM) observations. Sixty-four human teeth with class V caries that measured with DIAGNOdent were used. The samples were divided into four groups (n = 16) randomly according to caries removing methods of conventional method, Carisolv, Papacarie, and Er,Cr:YSGG laser system. The self-etch Clearfil SE Bond and Clearfil Majesty Es-II were used as restoration materials. After thermal cycles of 2,000 (5 and 55°C) samples were immersed in a 50% wt/wt AgNO3. Samples were finally imaged and scored under light microscopy and SEM, respectively. The data were statistically analyzed using Kruskal-Wallis H, pairwise comparison and Wilcoxon's T tests at 5% significance level (p < .05). Statistical analysis demonstrated that there was no significant difference between microleakage scores in dentin regions but there were significant difference between the Papacarie and Er,Cr:YSGGlaser in terms of leakage scores in enamel sites. No statistically significant difference in leakage scores emerged between light microscopy and SEM.

The Effects of Fresh Detox Juices on Color Stability and Roughness of Resin-Based Composites.

PURPOSE: To evaluate the effects of three fresh detox juices, including an orange, green, and red beverage, on the color stability and surface roughness of three anterior esthetic resin-based composites (RBCs). MATERIALS AND METHODS: Disk-shaped specimens were prepared with three different esthetic RBCs (Amaris, G-aenial Anterior, Clearfil Majesty ES-2) according to the manufacturers' instructions. Forty specimens were prepared for each RBC, and all specimens were stored in artificial saliva at 37°C for 24 hours. The initial color values and surface roughness measurements of the specimens were taken using a spectrophotometer and a profilometer. The specimens were then divided into 4 subgroups (n = 10). All specimens except the control specimens were immersed in their designated fresh detox juices (green, red, or orange) for 10 minutes twice a day. Color and surface roughness measurements were taken on day 15 and day 30, and the results were analyzed by one-way ANOVA and Tukey HSD test. The association between color change and surface roughness was evaluated by Spearman's Rank Correlation analysis. RESULTS: Color changes and surface roughness increased upon exposure to fresh detox juices for 15 and 30 days for all of the RBCs. All of the G-aenial and Amaris groups displayed color changes above the threshold of acceptability, whereas Clearfil Majesty ES-2 displayed a color change above the threshold of acceptability only after exposure to the red beverage for 30 days (ΔE > 3.7). With regard to surface roughness, Clearfil Majesty ES-2 outperformed the other RBCs (p < 0.001). According to Spearman's Rank Correlation analysis, there was no correlation between color change and surface roughness (p > 0.001). CONCLUSIONS: Exposure to the fresh detox juices used in this study led to similar color changes in the RBCs used in this study.

Effects of mouthrinses on discoloration of CAD-CAM manufactured restorative materials.

PURPOSE: To investigate the in vitro effect of four different mouthrinses on discoloration of popular CAD-CAM blocks. METHODS: 40 specimens were prepared and then were divided into five groups (n = 8) for each CAD-CAM block (Vitablocks Mark II, IPS e-max CAD, GC LRF, Enamic, Cerasmart). In total, four different mouthrinses for each group (Listerine Zero, Parodontax, Kloroben, Oral B Pro-Expert) and distilled water for the control group were used. The specimens were immersed in 20 ml solution for 2 minutes, twice a day for 21 days with a 12-hour interval. Color changes were measured with a digital spectrophotometer (Vita Easyshade) after 7, 14 and 21 days and specimens were prepared. For statistical analysis, color changes ( ΔE) were calculated and Kruskal Wallis and Wilcoxon tests were used. RESULTS: There was not a significant difference in ΔE1 and ΔE2 values (P> 0.005) while there was a significant one in ΔE3 (P= 0.001) after Bonferroni correction for CAD-CAM materials were carried out as it is independent of the mouthrinse. Listerine Zero brought about the most noticeable color change in all CAD-CAM materials except e.Max after 21 days. CLINICAL SIGNIFICANCE: Discoloration effect of the tested mouthrinses on various CAD-CAM materials was different depending on the usage period. However, these differences did not exceed the clinically perceptible values.

Dentinal tubule occluding capability of nano-hydroxyapatite; The in-vitro evaluation.

In this in-vitro study, the effectiveness of experimental pure nano-hydroxyapatite (nHAP) and 1%, 2%, and 3% F¯ doped nano-HAp on dentine tubule occlusion was investigated. And also, the cytotoxicity of materials used in the experiment was evaluated. Nano-HAp types were synthesized by the precipitation method. Forty dentin specimens were randomly divided into five groups of; 1-no treatment (control), 2-specimens treated with 10% pure nano-HAp and 3, 4, 5 specimens treated with 1%, 2%, and 3% F- doped 10% nano-HAp, respectively. To evaluate the effectiveness of the materials used; pH, FTIR, and scanning electron microscopy evaluations were performed before and after degredation in simulated body fluid. To determine cytotoxicity of the materials, MTT assay was performed. Statistical evaluations were performed with F and t tests. All of the nano-HAp materials used in this study built up an effective covering layer on the dentin surfaces even with plugs in tubules. It was found that this layer had also a resistance to degradation. None of the evaluated nano-HAp types were have toxicity. Fluoride doping showed a positive effect on physical and chemical stability until a critical value of 1% F- . The all evaluated nano-HAp types may be effectively used in dentin hypersensitivity treatment. The formed nano-HAp layers were seem to resistant to hydrolic deletion. The pure and 1% F- doped nano-HAp showed the highest biocompatibility thus it was assessed that pure and 1% F- doped materials may be used as an active ingredient in dentin hypersensitivity agents.

Evaluation of the Place of Chemo-Mechanical Caries Removal Method in Dental Education among Turkish Students

Objective: To evaluate the place of chemo-mechanical caries removal (CMCR) methods within the scope of undergraduate education of two different dental faculties which located in close geography but with different socio-economic characteristics. Material and Methods: In this cross-sectional, descriptive study totally 130 participants (Ankara: n = 78 and Kirikkale: n = 52) were evaluated. A survey which consisting of 14 questions were conducted by face to face. Data was analyzed using the SPSS software. Frequency distributions and the Chi-Square test were applied. Results: 66.9% of the participants reported that they had knowledge about the chemomechanical caries removal method. 60% had knowledge about the mechanism of CMCR. Thirty-six point two percent of the participants from Ankara stated that they had knowledge about the CMCR mechanism and 40% did not know about any of the methods of CMCR. 52.8% of the respondents indicated that they should be minimally invasive in choosing the CMCR method. The most important reason for not choosing the CMCR method was inadequate method (32%) and takes too much time (32%). Conclusion: Significant differences were found between the students of the two cities regarding the awareness of the CMCR method. In order to overcome this disparity, the curriculum place of the CMCR method needs to be determined precisely and clearly.

Sub-ablative Er,Cr:YSGG laser irradiation under all-ceramic restorations: effects on demineralization and shear bond strength.

This study evaluated the caries resistant effects of sub-ablative Er,Cr:YSGG laser irradiation alone and combined with fluoride in comparison with fluoride application alone on enamel prepared for veneer restorations. And also, evaluated these treatments' effects on the shear bond strength of all-ceramic veneer restorations. One hundred and thirty-five human maxillary central teeth were assigned to groups of 1a-control, 1b-laser treated, 1c-fluoride treated, 1d-laser + fluoride treated for shear bond testing and to groups of 2a-positive control(non-demineralised), 2b-laser treated, 2c-fluoride treated, 2d-laser + fluoride treated, 2e-negative control (demineralised) for microhardness testing (n = 15, N = 135). Demineralisation solutions of microhardness measurements were used for the ICP-OES elemental analysis. The parameters for laser irradiation were as follows: power output, 0.25 W; total energy density, 62.5 J/cm2 and energy density per pulse, 4.48 J/cm2 with an irradiation time of 20 s and with no water cooling. Five percent NaF varnish was used as fluoride preparate. ANOVA and Tukey HSD tests were performed (α = 5%). Surface treatments showed no significant effects on shear bond strength values (p = 0.579). However, significant differences were found in microhardness measurements and in elemental analysis of Ca and P amounts (p < 0.01). Surface-treated groups showed significantly high VNH values and significantly low ICP-OES values when compared with non-treated (-control) group while there were no significance among surface-treated groups regarding VHN and ICP-OES values. Sub-ablative Er,Cr:YSGG treatment alone or combined with fluoride is as an effective method as at least fluoride alone for preventing the prepared enamel to demineralization with no negative effect on shear bond strength.

Fracture Resistance of Incisal Fragments Following Reattachment With Different Techniques in Simulated Crown Fractures.

Introduction: The aim of the present study was to evaluate and compare the effect of acidetching and Er, Cr:YSGG laser treatment before the reattachment of incisal fragments in coronal fractures of permanent incisor teeth. Methods: Sixty-six sound human maxillary incisors were randomly divided into 3 groups (n=22). Teeth were embedded in self-cure acrylic resins by leaving one-third of the crowns out, and uncomplicated crown fractures were obtained using an Instron testing device. The fragments were reattached using 3 different procedures and a hybrid resin composite (Z250): Group I: Acid etching + Prime & Bond NT; Group II: Er,Cr:YSGG + Prime & Bond NT; Group III: Er,Cr:YSGG + Acid etching + Prime & Bond NT. The percentages of shear bond strengths were determined by comparing fracture strengths of sound and reattached teeth for 3 groups. All data were analyzed statistically with Kruskal-Wallis H test. Results: Mean fracture strengths of the reattached fragments were between 51.02% and 62.93% of that of the sound teeth in all groups. Group I had significantly higher percentages of shear bond strength values (P<0.05) when compared to group II and group III. No significant difference (P>0.05) was found between group II and group III. Conclusion: The findings of the present study show that Er,Cr:YSGG laser irradiation prior to the reattachment of incisal fragments has a negative effect on fracture strength.

Discoloration Effects of Traditional Turkish Beverages on different Composite Restoratives.

AIM: The aim of this study was to evaluate the discoloring effects of five beverages including, especially, traditional Turkish ones on five commonly used dental composites by using a spectrophotometer device. MATERIALS AND METHODS: Five methacrylate-based composites (shade A2) were selected to evaluate their color stability (175 disk samples). Four of them (Filtek Ultimate Universal, Clearfil Majesty ES-2, Tetric EvoCeram, and Cavex Quadrant Universal LC) were nanofilled universal composites for both anterior and posterior restorations, and one (Clearfil Majesty Posterior) was nano-superfilled for posterior restorations. The tested beverages were tamarind syrup, ottoman syrup, turnip juice, pomegranate juice, and distilled water (control). All samples were kept in an incubator at 37°C for 12 days (measured at 3rd, 6th, 12th day intervals) in immersion solutions which was equivalent to 1 year in vivo. Color measurements were made with VITA Easyshade Advance (Vident, Brea, CA) spectrophotometer device according to CIE L*a*b* system. Statistical analysis was performed with analysis of variance and least significant difference test to analyze differences in L*a*b* and ΔE values. RESULTS: All materials showed significant discoloration (p < 0.05) when compared with the control group. The highest ΔE was observed in turnip juice, whereas ottoman syrup had the lowest ΔE. Tetric EvoCeram showed the lowest ΔE, while Clearfil Majesty ES-2 showed the highest ΔE. CONCLUSION: In all the groups tested, clinically unacceptable ΔE values were obtained. Although color stability of methacrylate-based composites has been widely investigated, this has not been done before with these kinds of immersion solutions. CLINICAL SIGNIFICANCE: Potential discoloration might be limited by dietary adjustments based on in vitro evaluations.

Florid cemento-osseous dysplasia: A rare case report evaluated with cone-beam computed tomography.

A 29-year-old systemically healthy female patient presented to our department. Cone-beam computed tomographic images showed multiple well-defined sclerotic masses with radiolucent border in both right and left molar regions of the mandible. These sclerotic masses were surrounded by a thin radiolucent border. We diagnosed the present pathology as florid cemento-osseous dysplasia and decided to follow the patient without taking biopsy. For the patient, who did not have any clinical complaints, radiographic followupis recommended twice a year. The responsibility of the dentist is to ensure the follow-up of the diagnosed patients and take necessary measures for preventing the infections.

Evaluation of Novel Microabrasion Paste as a Dental Bleaching Material and Effects on Enamel Surface.

PURPOSE: Dental fluorosis is an enamel dysplasia that can cause serious aesthetic and psychological problems. An examination of the literature shows that the microabrasion method is the most effective way to treat fluorosis. This study aimed to produce a prototype microabrasion paste for the treatment of fluorosis stains and to examine the effects of the paste on tooth structure and color in vitro. MATERIAL AND METHODS: Forty extracted upper incisor teeth were used in this study. The teeth were divided into four treatment groups: a prototype microabrasion paste group (N = 10), an Opalustre paste group (N = 10), a 37% phosphoric acid gel group (N = 10), and a distilled water (control) group (N = 10). Following treatment, all specimens in each of the four groups were examined by scanning electron microscopy for changes in their surface enamel. Changes in tooth color changes of the 1st and 2nd groups were measured before and after treatment by a VITA Easyshade Advance spectrophotometer. RESULTS: In scanning electron microscopy evaluations, significant differences were not observed between the 1st and 2nd groups. Differences in three color-measuring coordinates between the 1st and 2nd were also not statistically significant (p > 0.05). CONCLUSIONS: The study demonstrated that the prototype paste is a viable treatment option for dental fluorosis. CLINICAL SIGNIFICANCE: The findings in this study demonstrated that the tested prototype microabrasion paste is extremely harmless, minimally invasive agent, and able to improve the color, shine and chrome values of the teeth.

The effect of temperature change on fluoride uptake from a mouthrinse by enamel specimens.

OBJECTIVE: The aim of this study was to examine the effect of temperature on fluoride uptake by enamel specimens from a 0.05% NaF-fluoridated mouthrinse (Oral-B Advantage; Oral-B Laboratories, Newbridge, UK). METHODS: Enamel specimens were prepared from extracted human maxillary central incisors. A fluoride-specific ion electrode was used to measure the uptake from a 2 ppm fluoride solution containing 50.0 mL of distilled water, total ion strength adjustment buffer, and fluoridated rinse at 3 different temperatures (room temperature, 25°C; human body temperature, 37°C; hyper-fever temperature, 43°C). One-way analysis of variance and least significant difference were used to assess intragroup and intergroup differences (P<.05). RESULTS: The study found that both the amount and the rate of fluoride uptake increased significantly with increase in temperature. This effect was particularly noticeable at 43°C. CONCLUSIONS: The temperature of the NaF mouthrinse may easily and safely be increased beyond room temperature by placing a container of the NaF mouthrinse in a bowl of hot water, allowing greater fluoride penetration into the enamel from the mouthrinse when used at home as a routine prophylactic agent.